Yes, for most markets. Overtime counts for Moneyline, Point Spread, Point Totals, and player stat markets unless the market says otherwise.
Overtime does not count for quarter markets or for Highest Scoring Half and Highest Scoring Quarter markets. Regulation Time Result (3-Way Moneyline) markets also exclude overtime.
For example: if a game finishes 110-110 after four quarters and your team wins 121-118 in overtime, a standard Moneyline bet on that team wins, and Point Totals are settled on the combined overtime score of 239.
For more information, see the General Rules, Tickets & Wagers article.